WebOct 9, 2013 · 10K views 9 years ago This time-lapse video compares the VENTANA BenchMark GX IHC/ISH automated staining instrument to manual processing and highlights key automation benefits such as less... WebJan 31, 2007 · b, f, and j show MICA/B staining of sections (red); c, g, and l display immunolabeling with CC1 (green), and d and h show an overlay of MICA/B and CC1, along with Hoechst nuclear stain. There is overlay of MICA/B and CC1 stains as seen by the orange-yellowish color on multiple cells (d, h); white arrows are examples of double …
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WebApplications Tested: This CC1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that … Weban alkaline buffer (CC1) and performed on the BenchMark Ultra, Ventana. A strong nuclear staining reaction is seen in virtually all follicular epithelial cells. No background staining is seen. Insufficient TTF1 staining of the thyroid gland using the mAb clone 8G7G3/1 (Ventana, RTU) with HIER in an alkaline buffer im in tune who
The oligodendrocyte-specific antibody ‘CC1’ binds …
WebDec 27, 2016 · The stroke core is demarcated as the region of loss of oligodendrocytes (CC1 stain) in adjacent sections. ( D) Undifferentiated reporter cells, NG2 + /YFP +, at 7 d after stroke (arrows). ( E) NG2 reporter positive cell stained for a maker of a more mature oligodendrocyte, CC1 (arrow). Washing buffer between the steps is Reaction buffer. Apply 100 µl volume of primary and secondary antibodies. 1. Tissue Sample, Paraffin. 2. Deparaffinization in EZ prep 75°C 8 minutes. 3. Cell Conditioning using Conditioner #1, Standard CC1, 95°C 44 minutes. 4. Block with Inhibitor CM, 37°C 4 minutes. 5. No Heat. 6. … See more Paraffin sections of 4 µm thickness are baked overnight at 50°C. The deparaffinization step is done in the Ventana Discovery XT platform using EZ prep solution. See more The standard antigen retrieval method is Heat Induced Epitope Retrieval (HIER) in Tris-EDTA buffer pH 7.8 at 95°C for 44 min (standard CC1) and is performed in the Ventana Discovery XT. If expected results are not … See more For immunohistochemistry, the following reagents are commercially available from Roche, Ventana Medical 1. Systems, Tucson, AZ, USA. 2. EZ Prep Solution, 950-100 3. Reaction … See more For blocking endogenous peroxides and protein, these steps are done in the Ventana Discovery XT platform using Inhibitor CM. … See more WebMaterial Safety Data Sheet PAGE : 1 OF 6 1. Identification of the substances/ mixture and of the company/ undertaking 1.1 Product Identifiers Product Code RM3490 im intuition\\u0027s