WebSaccharogenic methods, on the other hand, monitor the generation of reducing groups. Inasmuch as each hydrolytic cleavage induced by α-amylase creates a new reducing group, this increase is directly proportional to the enzymatic activity. References (57) A.C. Kibrick et al. J. Biol. Chem. (1951) V.C. Myers et al. J. Biol. Chem. (1944) WebThe major principles of assay on which the various methods of determining amylase activity are based are as follows: 1. Decrease in viscosity of a starch solution. 2. Decrease in turbidity of a starch suspension. 3. Diminution in the .starch-iodine color. 4. Increase in the reducing power of starch solution.
[PDF] A rapid saccharogenic method for the …
WebAbstract A modification of Somogyi's saccharogenic method for the determination of amylase activity has been presented for serum and urine. The starch substrate employed … WebApr 1, 1970 · The method is suitable for use with microsamples of all biological fluids, and is specific for the final products of starch digestion. Values found for sera from 55 apparently healthy blood donors ranged from 0.15 to 1.55 (mean, 0.83; standard deviation, ±0.4) mg of glucose per ml per h, corresponding to 7.5 to 78 Somogyi units. news 色
Infinity™ Amylase Liquid Stable Reagent - Fisher Sci
Webthe methods utilising insoluble substrates (correla tion coefficients varying between 0.91 and 0.98). Correlation was also good between the insoluble substrate methods and the saccharogenic method (correlation coefficients between 0.90 and 0.92). However, the starch-iodine method correlated poorly with both the insoluble substrate and WebAdd 10mL water and dissolve the sugars. Pipette out aliquots of 0.1 or 0.2mL to separate test tubes. Pipette out 0.2, 0.4, 0.6, 0.8 and 1mL of the working standard solution into a … WebApr 1, 1995 · General principles and methods of investiga-tions. Cliii Chem 1981; ... saccharogenic, or dye-coupled starch methods and are expected to gain popularity … news 訳