Web20 Jun 2024 · pip install google_trans_new Basic example. To translate a text from one language to another, you have to import the google_translator class from … WebReverse transcription was performed using 2.5 µl of aforementioned RT MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Consecutive, Takara Bio). cDNA was strengthened with 8 µl of the PCR MasterMix (SMART-Seq v5 Ultra Low Input RNA Kit for Sequencing, Takara Bio) with 25 cycles of amplification.
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WebReverse transcriptases (RTs) are RNA-directed DNA polymerases that synthesize a DNA copy (cDNA) from a target RNA. To apply PCR to the study of RNA, the RNA sample must first be reverse transcribed to cDNA to provide the … WebA Takara Bio Company 1290 Terra Bella Ave. Mountain View, CA 94043 Technical Support (US) E-mail: [email protected] www.clontech.com User Manual. ... Following reverse transcription, SMART ... shell sgbp
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Web31 Jan 2024 · Reverse transcription PCR (RT-PCR) is a popular method for detecting RNA viruses in plants. RT-PCR is usually performed in a classical two-step procedure: in the first step, cDNA is synthesized by reverse transcriptase (RT), followed by PCR amplification by a thermostable polymerase in a separate tube in the second step. WebDescription : PrimeScript II Reverse Transcriptase (RTase) yields high-quality cDNA with the least amount of background at standard reaction temperature (42℃) that doesn't cause RNA degradation because of its prominent primer use efficiency and addition of accessory protein, which resolves RNA secondary structure to inhibit cDNA synthesis and … WebThe High Capacity cDNA Reverse Transcription Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 µg total RNA. Reactions can be scaled up to 100 µL to … sporcle greek mythology